Chub-s7
WebMay 17, 2024 · Chub-S7 adipocytes have been used for studying cellular metabolism , adipogenic differentiation, and miRs regulation . Other … Web> chub s7 cells. micrococcal nuclease mnase About; News; Press Release; Team; Advisors; Partners; Contact; Bioz Stars; Bioz vStars; 86 : Buy from Supplier : Structured …
Chub-s7
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WebCellosaurus Chub-S7 (CVCL_0239) Doubling time: 45.6 +- 0.8 hours (PubMed= 12934077 ). Transfected with: UniProtKB; P03129; Human papillomavirus type 16 protein E7. … WebOct 20, 2024 · Chub-s7 cells were cultured until 24h post-confluence and differentiation was induced by adding 1mM dexamethasone, 0.5mM IBMX, 10mg/ml of insulin and 10mM of rosiglitazone to the medium for 14-21 days, until the appearance of adipocyte morphology. For quantitative real-time PCR experiments, differentiated chub-s7 adipocytes were …
WebJul 30, 2024 · We used Chub-S7, a human preadipocyte cell line that readily differentiates into adipocyte-like cells (Supplementary Fig. 1). First, given the proapoptotic action of … WebFeb 9, 2024 · A human adipocyte cell model, Chub-S7, was used to address the intracellular change in Lp-PLA2 in adipocytes. Results Lp-PLA2 and calcium-independent PLA2 (iPLA2) isoforms were altered by adiposity, …
WebOverexpression or siRNA-mediated knockdown of endogenous C/EBPalpha significantly increased or decreased, respectively, adiponectin mRNA levels in differentiated human Chub-S7 adipocytes, while neither C/EBPbeta nor C/EBPdelta significantly affected adiponectin expression in mature adipocytes. WebNov 1, 2013 · The Chub-S7 cell line (Nestlé Research Centre, Lausanne, Switzerland) was derived from human subcutaneous adipose tissue by coexpression of human telomerase reverse transcriptase and papillomavirus E7 oncoprotein (HPV-E7) genes ( 14) and has previously been characterized in detail showing expression of typical white adipose …
WebThe Chub-S7 cell line was derived from human subcutaneous primary preadipocytes which were transfected with human papillomavirus E7 oncoprotein and the human telomerase …
WebMillipore 7 15 d s7 nkx6 1 insulin mafa cells. 7 15 D S7 Nkx6 1 Insulin Mafa Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … puree french friesWebOct 14, 2011 · The Chub-S7 cell line is a transformed human subcutaneous pre-adipocyte cell line derived from an obese female that has been validated as a good model of … section 14 csspWebNov 19, 2024 · Human pre-adipocytes cell line (Chub-S7) and primary adipocytes were obtained from lean, obese and morbid obese patients, grown to confluence, differentiated for one week, maintained in nutrition media for next 7 days (day 14) and then used for further experimental analysis. In order to analyse B12 deficiency effects, customized media with ... section 14 cyfaWebNov 25, 2024 · The aim of our study was to use a combined epigenomic and transcriptomic approach to identify novel genes mediating the effect of B12 on adipogenesis.Human pre … section 14 criminal procedure scotland actWebMethods: ER stress was induced in post-differentiated Chub-S7 (AbdSc Ad) using tunicamycin (Tn) (0.25 μg/ml, 0.75 μg/ml) for 24 hrs, 48 hrs and 72 hrs. Assessment of mitochondrial function post Tn treatment was undertaken using the Extracellular Flux Analyser – evaluating oxygen consumption rate (OCR) and proton excretion (glycolysis ... section 14d building actWebJul 1, 2013 · The non-differentiated and differentiated control Chub-S7 cells presented similar rate of routine respiration (33.65 vs. 33.96 pmol/s × ml, respectively). The … section 14d children act 1989Web(Chub-S7) and human primary preadipocytes, we have defined the role of glucocorticoids and 11b-HSD1 in regulating adipose tissue differentiation. Human cells were differentiated with 1.0 mM cortisol (F), or cortisone (E) with or without 100 nM of a highly selective 11b-HSD1 inhibitor PF-877423. 11b-HSD1 mRNA expression increased across puree friet