Hif1 troublesome western blot
WebHIF-1α induces glycolytic reprograming in tissue-resident alveolar macrophages to promote cell survival during acute lung injury. View PDF. In eLife on 13 July 2024 by Woods, P. S., Kimmig, L. M., et al.. WebWestern blot analysis of HIF1A in A) HepG2 whole cell lysate, (untreated), B) HepG2 whole cell lysate, (200 µM CoCl2 treatment for 24 hr), C) HepG2 whole cell lysate, (500 µM CoCl2 treatment for 24 hr) using HIF1A polyclonal antibody (Product # PA5-85494) using 30 µg of sample at a dilution of 1:5000.
Hif1 troublesome western blot
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WebObjective: To investigate the relationship between hypoxia inducible factor 1 (HIF1α) and Wilms' tumor 1associating protein (WTAP) expression level in t(8;21) acute myeloid … WebCollect cells into PBS by centrifugation (non-adherent cells) or scraping from culture flasks followed by centrifugation (adherent cells). Wash cells 2X with ice cold PBS at 1000 x g …
Web27 de mar. de 2012 · RT-PCR and Western blot analysis indicated that hypoxia could up-regulate the expression of HIF-1α on both mRNA and protein levels. Hypoxic … WebDoes anyone have any advice on how to successfully extract HIF1a and HIF2a from HEK293T for measurement in a western blot. These are the materials I am using: …
Web15 de mar. de 2015 · The expression of PI3K, Akt, HIF1α, PARP, PKM2 and COX in gastric cancer cells was detected by immunofluorescence and Western blot analysis. We found that HIF1α and PKM2 protein expression levels were higher in advanced gastric cancer tissues than in gastritis tissues. WebHIF-1 alpha Antibody (H1alpha67) [NB100-105]: Western blot (WB) analysis of lysates from Caki-1 cells which were left un-treated or were treated with 100-300 uM of CoCl 2 for 4-6 hours. The antibody detected …
WebIn Western blotting, I detected clear lower molecular band in about 40-80kDa. On the other hand, Higher molecular band was also detected. I studied that theoretical MW of HIF-1 …
WebIn Western blotting, I detected clear lower molecular band in about 40-80kDa. On the other hand, Higher molecular band was also detected. I studied that theoretical MW of HIF-1 … graphic design art softwareWebMA1-515 detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT) from human, mouse, rat, and primate tissues. MA1-515 has been successfully used in Western blot, immunofluorescence, gel shift, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an 87 kDa protein representing ARNT in COS cell ... graphic design art showsWebWestern Blot tips. HIF-1 alpha can be a challenging target to work with in western blot. HIF-1 alpha is only stabilized at O2 concentrations below 5%. Under normoxic … chip xp modeWebMA1-515 detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT) from human, mouse, rat, and primate tissues. MA1-515 has been successfully used in Western blot, … graphic design art piecesWeb14th Aug, 2016. Ahmed E. Dhamad. Hi, Try to to detect really known protein like Actin or Tubulin to make sure your SDS-PAGE is good. Then try to load more protein 60-80ug "if … chip x readerWebImmunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309). Western blot analysis was performed using HIF1 alpha … graphic design art pngWeb14th Aug, 2016. Ahmed E. Dhamad. Hi, Try to to detect really known protein like Actin or Tubulin to make sure your SDS-PAGE is good. Then try to load more protein 60-80ug "if you have that much ... chipy and friend twitter