Web29 Jan 2024 · Denaturation Bubble-mediated Strand Exchange Amplification (SEA) was established, which is an isothermal method with only a primer pair and one Bst DNA … Web23 Feb 2015 · Loop-mediated isothermal amplification (LAMP) of DNA is a powerful isothermal nucleic acid amplification method that can generate upward of 109 copies from less than 100 copies of template DNA within an hour. Unfortunately, although the amplification reactions are extremely powerful, real-time and specific detection of LAMP …
Highly sensitive visual detection of nucleic acid based on a …
Web2 Feb 2024 · Denaturation bubble-mediated strand exchange amplification (SEA) is a novel constant temperature nucleic acid amplification method [ 18] using Bst DNA polymerase as same as LAMP. The DNA duplex dynamically dissociated, and the local base pairs opened to form the denaturation bubble. Web14 Sep 2024 · With good amplification efficiency for short-sequence targets, ESEA is an ideal choice for the point-of-care testing of SARS-CoV-2 with a high mutation rate. ESEA reaction can be completed in one step and verified by restriction enzyme digestion. The design consisting of three working primers greatly improved the amplification efficiency. skyscraper tapachula
[PDF] Rapid DNA detection and one-step RNA detection catalyzed …
Web1 Apr 2024 · Here, a simple denaturation bubble-mediated strand exchange amplification method (SEA) requiring only a pair of primers and one polymerase was first reported for … Web11 Feb 2024 · Salmonella is one of the most common pathogens associated with food-borne illness resulting from seafood consumption. Herein, an accelerated strand exchange amplification (ASEA) requiring only a pair of primers and one polymerase was first reported for ultra-fast, one-step RNA amplification detection of Salmonella in seafood. The ASEA … Web15 Aug 2024 · Strand exchange amplification (SEA) is a simple isothermal amplification method [ 32 ], it takes advantage of the DNA duplex's dynamically dissociate at appropriate reaction temperature, leading the local opening of base pairs to create a single-stranded denaturation bubble [ [33], [34], [35]]. skyscraper sunflower growing